Gramnegative bacteria can be responsible for infections including woundsurgical site infections, pneumonia and meningitis. Unrooted phylogenetic tree based on a comparison of the 16s ribosomal dna sequence of pantoea agglomerans lma2 gq478021 and some of their closest phylogenetic relatives validly published strains, the tree was created by the neighborjoining method, the numbers on the tree indicates the percentages of bootstrap sampling derived from 1,000 replications, bar inferred. It is primarily an environmental and agricultural organism that inhabits plants, soil, and water. Pantoea agglomerans is reported as the most common organism isolated after such injuries, in the scarce reports available worldwide. Pdf pantoea agglomerans, a plant pathogen causing human. The three nondiazotrophic isolates were identified as strains of enterobacter spp. Frontiers a carotenoiddeficient mutant in pantoea sp. Pdf the large universal pantoea plasmid lpp1 plays a. Pantoea was delineated as a genus approximately 25 years ago, but since then, approximately 20 species have been identified having a diversity of characteristics. Enterobacteriaceae to plants by lygus hesperus hemiptera. Pantoea dispersa bacteremia in an immunocompetent patient. The locusts have adapted to use the guaiacol produced by pantoea agglomerans to initiate the synchronized swarming of locusts. A diagnostic multiplex pcr scheme for identification of.
It is regarded as an emerging pathogen based on the increasing number of reports of diseases occurring on previously unrecorded hosts in. Nevertheless, it is not possible to rule out the possibility that, at least in some cases, accidental microbial contamination may have occurred coming from staff andor processing plant. They are also ubiquitous occurring in diverse ecological niches. Pantoea agglomerans is a gramnegative aerobic bacillus that belongs to the family enterobacteriaceae. Pantoea ananatis is the most wellstudied of the pantoea species on onion. Pantoea agglomerans, a bacterium associated with plants, is not an obligate infectious agent in humans. Nevertheless, this species has also also been identified as a cause of diseases in a range of cultivable plants, such as cotton, sweet onion, rice, maize, sorghum, bamboo, walnut, an ornamental plant called chinese taro alocasia cucullata. Verma 2 1 department of plant biology, rutgers university, 59 dudley rd. It is commonly found in the ecological niches such as water, soil, sewage, seeds, vegetables, feculent material and foodstuffs, as well as reported as both commercial and opportunistic pathogens of animals and. Pantoea agglomerans e325, the active ingredient in a commercial product for fire blight control, was previously shown in vitro to produce a. Some strains have proven effective as biological control agents and plantgrowth promoters, while other strains have been implicated in diseases of a broad range of plant hosts and humans.
Prevelance and antibiotic susceptibility patterns of pantoeaspp. Pantoea ananatis causes disease symptoms in a wide range of economically important agricultural crops and forest tree species worldwide. Here, we present the genome sequence of pantoea sp. A variety of species were collected from nonsurface sterilized p.
Pantoea spp in addition, there are very little information regarding the role ofpantoea spp in human bacterial infections. Despite their prevalence, little is known about their association with plants, particularly where they occur as pathogens. Pantoea bacteria are yellow pigmented, ferment lactose, are motile, and form mucoid colonies. All species of the genus pantoea can be isolated from feculent material, plants, and soil, where they can be either pathogens or commensals. Effects of postharvest onion curing parameters on bulb rot. Pantoea is a genus of gramnegative bacteria of the family enterobacteriaceae, recently separated from the genus enterobacter. Pdf seed transmission of pantoea stewartii in field and. Highquality draft genome sequence of a new phytase. During the period from december 2014 to july 2015, 40 isolates ofpantoea spp. Pantoea agglomerans is a gramnegative rod in the enterobacteriaceae family. Bible an, fletcher sj, pelletier da, schadt cw, jawdy ss, weston dj, engle ln, tschaplinski t, masyuko r, polisetti s, bohn pw, coutinho ta, doktycz mj and morrellfalvey jl 2016 a carotenoiddeficient mutant in pantoea sp. The large universal pantoea plasmid lpp1 plays a major role in biological and ecological diversification. Pantoea grimont major reference works wiley online.
Within the genus pantoea, the most prominent species and most common hospital isolate is p. Draft genome sequence of pantoea ananatis strain lmg 2665t, a bacterial pathogen of pineapple fruitlets. Pantoea agglomerans is found in the gut of locusts. Isolates from water and soil have been harnessed for industrial purposes including bioremediation, and the. We present a 34yearold man who was presented for the management of diabetic ketoacidosis and developed pantoea agglomerans bacteremia after one week of hospitalization. Pantoea agglomerans is an aerobic bacterium, so it requires a certain level of air circulation in order to survive. Prevelance and antibiotic susceptibility patterns of. However, some isolates of pantoea and other bacteria such as sphingomonas have similar biochemical and morphological features on common culture media and are thus difficult to isolate selectively and accurately diagnose. Isolation, identification and antimicrobial susceptibility. Antibiosis activity of pantoea agglomerans biocontrol strain e325 against erwinia amylovora on apple flower stigmas. Ngsbased phylogeny of diphtheriarelated pathogenicity. Pantoea agglomerans strain eh318 produces two antibiotics. Some species show quorum sensing ability that could drive different gene expression, hence controlling certain physiological activities.
Pantoea agglomerans infection behaving like a tumor after. This bacterium has been reported as both commensal and pathogen of animals and humans 1. Pantoea is a genus of gramnegative bacteria of the family erwiniaceae, recently separated from the genus enterobacter. As they are highly and increasingly resistant to multiple drugs, preventing the spread of these organisms is a key goal in the healthcare setting. Pdf draft genome sequence of pantoea ananatis strain lmg. The bacterial genus pantoea comprises many versatile species that have been isolated from a multitude of environments. A multiplex pcr protocol which can distinguish these three species and also detects members of other pantoea species was further developed. The large universal pantoea plasmid lpp1 plays a major. Associated with smooth crabgrass digitaria ischaemum seed inhibit competitor plant species matthew t. Enterobacteriaceae were identified from liquid diet that was styletprobed and fed on by adult lygus cooper et al. We aimed to investigate if differences between species can be observed. Isolation of endophytic diazotroph pantoea agglomerans and. Nosocomial infection of pantoea spp in neonatal unit about. Pantoea agglomerans formerly enterobacter agglomerans is a gramnegative aerobic bacillus in the family enterobacteriaceae.
Darweesh department of biology, college of sciences, kufa university, najaf, iraq. Some strains are or have been thought to be phytopathogenic. Pantoea agglomerans, a plant pathogen causing human. Pantoea dispersa it the next emerging monster in our. Pantoea ananatis is found in a wide range of natural environments, including water, soil, as part of the epi and endophytic flora of various plant hosts, and in the insect gut. Pantoea agglomerans, a plant pathogen causing human disease.
However, it could be however, it could be a cause of opportunistic human infections, mostly by wound infection with plant material, or as a hospitalacquired infection. Genomics and current genetic understanding of erwinia. Modeling the rate of attachment of listeria monocytogenes. The reason for hospitalization was an early respiratory distress in 60% of cases. Pantoea agglomerans usually occurs in plants as an epi or endophytic symbiont, often as mutualist. We characterized 36 pantoea strains, including 28 strains of diverse origins initially identified as p. The genus pantoea is a rare pathogen in a clinical setting, and is divided into 20 different species such as pantoea agglomerans, pantoea ananatis, pantoea deleyi. Allen1,2 department of pediatrics, sections of infectious disease1 and. Antibiosis activity of pantoea agglomerans biocontrol. Erwinia herbicola strain eh318 produces through antibiosis a complex zone of inhibited growth in an overlay seeded with erwinia amylovora, the causal agent of fire blight.
Colonization of the cut surface is a twostage process. Phylogeny and identification of pantoea species and typing. Seed transmission of pantoea stewartii was evaluated by assays of more than 76,000 plants in greenhouse and field growout trials. Genomics and current genetic understanding of erwinia amylovora and the fire blight antagonist pantoea vagans, trees, 2012, pp. A semiselective medium to isolate and identify bacteria. This zone is caused by two antibiotics, named pantocin a and b. Using a genomic library of eh318, two cosmids, pcpp702 and pcpp704, were identified that conferred on escherichia coli the ability. Two other genera, known as tatumella and erwinia, have close association with pantoea, all three clubbed together in the family enterobacteriaceae along with other groupsgenera such as citrobacter, cronobacter, enterobacter, escherichia more details, klebsiella, and salmonella more details. In this paper, we summarize the current status of leaf blight caused by pantoea spp. Result using 34 whole genome sequences of the threemajor plantpathogenic pantoea species, a set of pcr primers that specifically detect each of the three species, p. Pantoea infections usually begin as watersoaked lesions on the leaves that progress down the neck into the developing bulb causing a disease commonly known as centre rot conn et al. Pantoea is a gramnegative, nonencapsulated, nonsporeforming, ubiquitous straight rod which can be isolated from geographical and ecological sources such as plant surfaces, buckwheat seeds, human feces, and the environment. It is reported as both commensal and opportunistic pathogen of animals and humans.
So, this study was conducted to isolate and identifypantoea spp from different sources clinical and environmental by different methods and detect the susceptibility of bacteria to different type of antibiotic. Pantoea agglomerans, an anaerobic gramnegative bacillus, is a rare cause of opportunistic infections affecting premature infants to seniors. Isolation and characterization of plant growth promoting. Pantoea agglomerans and other pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of pantoea strains and their possible association with hosts and disease remain poorly known, and identification of pantoea species is difficult. Yr343, a bacteria isolated from the rhizosphere of populus deltoides, is defective in root colonization. Characterization of two pantoea strains isolated from. First report from iran jalal mardaneh 1, 2 and mohammad mehdi soltan dallal 1, 3. Isolation, identification and antimicrobial susceptibility of pantoea enterobacter agglomerans isolated from consumed powdered infant formula milk pif in nicu ward.
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